HeLa cells were subjected to SDS PAGE followed by western blot with 16690-1-AP (ALY antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16690-1-AP (ALY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16690-1-AP (ALY antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
WB result of ALY antibody (16690-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ALY transfected HeLa cells.